来源 用途 交叉反应性 理论分子量 实际分子量 Rabbit WB, IF H, M, R 39/42kDa 43kDa WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing. H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected. 配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。 建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)： WB IP IF IHC ICC FC ChIP ChIP-seq 1:500-1:2000 - 1:50-1:100 - - - - - 抗体详细信息如下: About this Antibody Name SETD8 Rabbit Polyclonal Antibody Category Rabbit Polyclonal Antibody (pAb); Primary antibody Isotype IgG Purification method Affinity purification Positive samples A-549, 293T, Mouse kidney Cellular location Chromosome, Nucleus Customer validation - About the Immunogen Immunogen Recombinant fusion protein of human SETD8 (NP_065115.3). Sequence ENSVTHHEVKCQGKPLAGIYRKREEKRNAGNAVRSAMKSEEQKIKDARKGPLVPFPNQKSEAAEPPKTPPSSCDSTNAAIAKQALKKPIKGKQAPRKKAQGKTQQNRKLTDFYPVRRSSRKSKAELQSEERKRIDELIESGKEEGMKIDLIDGKGRGVIATKQFSRGDFVVEYHGDLIEITDAKKREALYAQDPSTGCYMYYFQYLSKTYCVDATRETNRLGRLINHSKCGNCQTKLHDIDGVPHLILIASRDIAAGEELLYDYGDRSKASIEAHPWLKH Gene ID 387893 Swiss Prot Q9NQR1 Synonyms KMT5A; PR-Set7; SET07; SET8; SETD8; lysine methyltransferase 5A Category Histone Methylation; Epigenetics; Crosstalk Between Protein Modifications Background SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins. SET8 is a single-subunit enzyme that mono-methylates histone H4 on Lys20, preferably on nucleosomal substrates. SET8 protein levels and Histone H4 Lys20 methylation are cell cycle regulated, both increasing in S phase and peaking at G2/M phase. SET8 interacts with the PCNA protein, associates with sites of active DNA synthesis, and is required for DNA replication and genome stability during S phase. Inhibition of SET8 using shRNA or siRNA results in arrest of replication forks, induction of double-stranded DNA breaks, and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Furthermore, SET8 methylates p53 on Lys382, down regulating the pro-apoptotic and checkpoint activation functions of p53. In response to DNA damage, SET8 expression levels decrease, allowing p53 to activate checkpoints and/or apoptosis. Both the methylation of histone H4 Lys20 and p53 appear to be important for the functions of SET8 in S phase. 包装清单： 产品编号 产品名称 包装 AF7971 SETD8 Rabbit Polyclonal Antibody 50μl AZ050 Western一抗稀释液 50ml — 说明书 1份 保存条件： SETD8 Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项： 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。 回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 为了您的安全和健康，请穿实验服并戴一次性手套操作。